The JIS Z 2801 standard (also known as the “film contact method”) is used to test the antimicrobial effectiveness of hard, non-porous surfaces such as plastics, metals and ceramics.

Test for antimicrobial efficacy according to JIS Z 2801

The JIS Z 2801 (“film contact method”) evaluates the antimicrobial activity of hard, non-porous surfaces and is used worldwide. The process has numerous real-life applications, from hospitals/clinics to manufacturers of consumer goods and household items. In addition to test samples (potentially equipped with antimicrobial agents), reference samples (test specimens without antimicrobial equipment or laboratory controls) are included.

Samples of a defined size are inoculated with a specific volume of a standardized bacterial cell suspension of Staphylococcus aureus and Escherichia coli. To avoid water loss on the sample surface, it is covered with a foil (film) after inoculation. The foil also has the task of distributing the inoculum evenly on the test sample and bringing the test organisms into close contact with the surface.

The test is performed in triplicate for each test sample and reference. After the specific contact times (0 h and 24 h), the microorganisms are eluted from the samples and the live cell counts per sample are determined. Finally, the values of the test samples are compared with those of the reference samples and the percentage bacterial reduction or the specific reduction factors are determined as values for the antibacterial efficacy.


  • JIS (Japanese Industrial Standard) Z 2801
  • ISO 22196

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