{"id":114257,"date":"2024-01-08T10:23:55","date_gmt":"2024-01-08T09:23:55","guid":{"rendered":"https:\/\/webserver.gfps.de\/sequencing\/"},"modified":"2024-06-14T19:06:10","modified_gmt":"2024-06-14T17:06:10","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/www.gfps.de\/en\/sequencing\/","title":{"rendered":"Sequencing"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;60&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;0&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-nhtu&#8221; back_image=&#8221;112741&#8243; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-983180&#8243; overlay_alpha=&#8221;51&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; enable_bottom_divider=&#8221;default&#8221; bottom_divider=&#8221;gradient&#8221; shape_bottom_h_use_pixel=&#8221;true&#8221; shape_bottom_height_percent=&#8221;100&#8243; shape_bottom_color=&#8221;accent&#8221; shape_bottom_opacity=&#8221;90&#8243; shape_bottom_index=&#8221;0&#8243; row_height_pixel=&#8221;280&#8243; row_name=&#8221;Intro&#8221; uncode_shortcode_id=&#8221;149784&#8243; back_color_type=&#8221;uncode-palette&#8221; shape_bottom_color_type=&#8221;uncode-palette&#8221; overlay_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; position_horizontal=&#8221;left&#8221; position_vertical=&#8221;bottom&#8221; gutter_size=&#8221;1&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; style=&#8221;dark&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; align_medium=&#8221;align_left_tablet&#8221; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_left_mobile&#8221; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; animation_speed=&#8221;1000&#8243; width=&#8221;1\/1&#8243; uncode_shortcode_id=&#8221;649805&#8243;][vc_custom_heading heading_semantic=&#8221;h3&#8243; text_font=&#8221;font-136269&#8243; text_size=&#8221;h4&#8243; text_weight=&#8221;300&#8243; text_transform=&#8221;uppercase&#8221; text_space=&#8221;fontspace-210350&#8243; sub_lead=&#8221;yes&#8221; sub_reduced=&#8221;yes&#8221; uncode_shortcode_id=&#8221;532112&#8243;]Identifications[\/vc_custom_heading][vc_custom_heading heading_semantic=&#8221;h1&#8243; text_size=&#8221;fontsize-155944&#8243; text_weight=&#8221;800&#8243; text_transform=&#8221;uppercase&#8221; text_space=&#8221;fontspace-111509&#8243; sub_lead=&#8221;yes&#8221; sub_reduced=&#8221;yes&#8221; uncode_shortcode_id=&#8221;730412&#8243;]Sequencing[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;0&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;0&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;0&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; style=&#8221;inherited&#8221; uncode_shortcode_id=&#8221;106173&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; style=&#8221;light&#8221; back_color=&#8221;color-lxmt&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; align_medium=&#8221;align_left_tablet&#8221; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_left_mobile&#8221; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; animation_delay=&#8221;200&#8243; width=&#8221;3\/4&#8243; uncode_shortcode_id=&#8221;116340&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_column_text uncode_shortcode_id=&#8221;105146&#8243;]As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.[\/vc_column_text][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; overlay_alpha=&#8221;0&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_visibility=&#8221;yes&#8221; medium_width=&#8221;0&#8243; mobile_visibility=&#8221;yes&#8221; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; animation_delay=&#8221;400&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;1\/4&#8243; uncode_shortcode_id=&#8221;184491&#8243;][\/vc_column][\/vc_row][vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;55&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;0&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-nhtu&#8221; overlay_alpha=&#8221;0&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; style=&#8221;inherited&#8221;][vc_column column_width_percent=&#8221;100&#8243; position_vertical=&#8221;middle&#8221; align_horizontal=&#8221;align_center&#8221; gutter_size=&#8221;3&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; back_color=&#8221;color-rgdb&#8221; back_image=&#8221;112742&#8243; back_position=&#8221;center center&#8221; overlay_alpha=&#8221;0&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; medium_width=&#8221;0&#8243; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; animation_delay=&#8221;400&#8243; zoom_width=&#8221;0&#8243; zoom_height=&#8221;0&#8243; width=&#8221;1\/4&#8243; uncode_shortcode_id=&#8221;318159&#8243; back_color_type=&#8221;uncode-palette&#8221; mobile_height=&#8221;300&#8243;][\/vc_column][vc_column column_width_percent=&#8221;100&#8243; gutter_size=&#8221;2&#8243; override_padding=&#8221;yes&#8221; column_padding=&#8221;4&#8243; style=&#8221;light&#8221; back_color=&#8221;color-xsdn&#8221; overlay_alpha=&#8221;50&#8243; shift_x=&#8221;0&#8243; shift_y=&#8221;0&#8243; shift_y_down=&#8221;0&#8243; z_index=&#8221;0&#8243; align_medium=&#8221;align_left_tablet&#8221; medium_width=&#8221;0&#8243; align_mobile=&#8221;align_left_mobile&#8221; mobile_width=&#8221;0&#8243; css_animation=&#8221;bottom-t-top&#8221; animation_delay=&#8221;200&#8243; width=&#8221;3\/4&#8243; uncode_shortcode_id=&#8221;188419&#8243; back_color_type=&#8221;uncode-palette&#8221;][vc_custom_heading text_weight=&#8221;800&#8243; text_transform=&#8221;uppercase&#8221; text_space=&#8221;fontspace-111509&#8243; text_uppercase=&#8221;&#8221; text_align=&#8221;text-left&#8221; uncode_shortcode_id=&#8221;926762&#8243;]Sequencing[\/vc_custom_heading][vc_column_text uncode_shortcode_id=&#8221;616857&#8243;]Especially when it comes to the causes of contamination in the manufacturing process, storage and transportation of medical devices. Reliable identification of the microorganism is also a decisive advantage in the troubleshooting process.<\/p>\n<p>This means safety for manufacturers and patients.<\/p>\n<p>Classical identification methods are based on the differentiation of the microorganism on the basis of morphological and physiological aspects, whereby the cell or colony is considered as a whole.<\/p>\n<p>At the molecular level, in addition to phenotypic analysis (proteome spectrum) using a mass spectrometer (MALDI TOF), we also have the option of genotypic analysis using Sanger sequencing.<\/p>\n<p>The genetic material of the cell as an analyte offers a well-founded information content and the use of GMP-validated databases enables maximum validity of the sequencing results with subsequent plausibility checks.[\/vc_column_text][vc_empty_space empty_h=&#8221;1&#8243;][vc_separator sep_color=&#8221;color-983180&#8243; uncode_shortcode_id=&#8221;107261&#8243; sep_color_type=&#8221;uncode-palette&#8221;][vc_empty_space empty_h=&#8221;1&#8243;][vc_column_text uncode_shortcode_id=&#8221;198159&#8243;]<strong>Guidelines:<\/strong><\/p>\n<ul>\n<li>Ph. Eur. 2.6.2 Nucleic Acid Amplification Techniques (5.4 Detection)<\/li>\n<li>USP &lt;1126&gt; Nucleic acid-based techniques-Extraction, Detection and Sequencing.<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_empty_space empty_h=&#8221;1&#8243;][vc_separator sep_color=&#8221;color-983180&#8243; uncode_shortcode_id=&#8221;107261&#8243; sep_color_type=&#8221;uncode-palette&#8221;][vc_empty_space empty_h=&#8221;1&#8243;][vc_column_text uncode_shortcode_id=&#8221;112133&#8243;]We are happy to support you with your sequencing questions at <a href=\"mailto:freigabe@gfps.de\"><br \/>\n  <strong>freigabe@gfps.de<\/strong><br \/>\n<\/a>.[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row unlock_row_content=&#8221;yes&#8221; row_height_percent=&#8221;60&#8243; override_padding=&#8221;yes&#8221; h_padding=&#8221;0&#8243; top_padding=&#8221;0&#8243; bottom_padding=&#8221;0&#8243; back_color=&#8221;color-nhtu&#8221; back_image=&#8221;112741&#8243; back_position=&#8221;center center&#8221; overlay_color=&#8221;color-983180&#8243; overlay_alpha=&#8221;51&#8243; equal_height=&#8221;yes&#8221; gutter_size=&#8221;0&#8243; column_width_percent=&#8221;100&#8243; shift_y=&#8221;0&#8243; z_index=&#8221;0&#8243; enable_bottom_divider=&#8221;default&#8221; bottom_divider=&#8221;gradient&#8221; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-114257","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Sequencing - GfPS<\/title>\n<meta name=\"description\" content=\"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.gfps.de\/en\/sequencing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sequencing - GfPS\" \/>\n<meta property=\"og:description\" content=\"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.gfps.de\/en\/sequencing\/\" \/>\n<meta property=\"og:site_name\" content=\"GfPS\" \/>\n<meta property=\"article:modified_time\" content=\"2024-06-14T17:06:10+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.gfps.de\/en\/sequencing\/\",\"url\":\"https:\/\/www.gfps.de\/en\/sequencing\/\",\"name\":\"Sequencing - GfPS\",\"isPartOf\":{\"@id\":\"https:\/\/www.gfps.de\/en\/#website\"},\"datePublished\":\"2024-01-08T09:23:55+00:00\",\"dateModified\":\"2024-06-14T17:06:10+00:00\",\"description\":\"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.gfps.de\/en\/sequencing\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.gfps.de\/en\/sequencing\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.gfps.de\/en\/sequencing\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Startseite\",\"item\":\"https:\/\/www.gfps.de\/en\/home\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Sequencing\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.gfps.de\/en\/#website\",\"url\":\"https:\/\/www.gfps.de\/en\/\",\"name\":\"GfPS\",\"description\":\"Gepr\u00fcfte Qualit\u00e4t f\u00fcr Ihre Medizinprodukte\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.gfps.de\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Sequencing - GfPS","description":"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.gfps.de\/en\/sequencing\/","og_locale":"en_US","og_type":"article","og_title":"Sequencing - GfPS","og_description":"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.","og_url":"https:\/\/www.gfps.de\/en\/sequencing\/","og_site_name":"GfPS","article_modified_time":"2024-06-14T17:06:10+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"4 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.gfps.de\/en\/sequencing\/","url":"https:\/\/www.gfps.de\/en\/sequencing\/","name":"Sequencing - GfPS","isPartOf":{"@id":"https:\/\/www.gfps.de\/en\/#website"},"datePublished":"2024-01-08T09:23:55+00:00","dateModified":"2024-06-14T17:06:10+00:00","description":"As an institute with different requirements in the field of microbiology, we are frequently confronted with questions about error analysis.","breadcrumb":{"@id":"https:\/\/www.gfps.de\/en\/sequencing\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.gfps.de\/en\/sequencing\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.gfps.de\/en\/sequencing\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Startseite","item":"https:\/\/www.gfps.de\/en\/home\/"},{"@type":"ListItem","position":2,"name":"Sequencing"}]},{"@type":"WebSite","@id":"https:\/\/www.gfps.de\/en\/#website","url":"https:\/\/www.gfps.de\/en\/","name":"GfPS","description":"Gepr\u00fcfte Qualit\u00e4t f\u00fcr Ihre Medizinprodukte","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.gfps.de\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/pages\/114257","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/comments?post=114257"}],"version-history":[{"count":2,"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/pages\/114257\/revisions"}],"predecessor-version":[{"id":114259,"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/pages\/114257\/revisions\/114259"}],"wp:attachment":[{"href":"https:\/\/www.gfps.de\/en\/wp-json\/wp\/v2\/media?parent=114257"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}